C12N 15/65, C12N 15/79, C12N 1/10, C12N 13/00, C12N 15/87, C12N 2830/50, A61K 39/002, A61K 2039/522, A61K 2039/51
Disclosed herein are expression vectors suitable for transfection in amoebas. The vectors may include a promoter from a protein-encoding gene from an amoeba, a selection marker, and a polynucleotide sequence encoding a polypeptide of interest, operably linked to the promoter. The promoter may be from the ACT1 gene from Naegleria fowleri.
Seyfang, Andreas; Massengill, Christopher L.; and Sievers, Sara R., "Transfection vector for pathogenic amoebae and uses thereof" (2019). USF Patents. 985.
University of South Florida