Document Type
Patent
Publication Date
November 2006
Patent Number
7132446
Abstract
Certain proanthocyanidins found in various wines, grape juice, and other plant extracts exhibit endothelium-dependent vasorelaxing activity that involves increased nitric oxide production by endothelial cells. The smallest procyanidins (PCs) possessing substantial EDR activity were isolated from grape seeds. A dimer di-gallate (epicatechin-galloyl-epicatechin-gallate) was purified from concord grape seed extracts by Toyopearl TSK-40 gel chromatography, HPLC and electrospray FTMS. Further characterization was achieved using tannase treatment and acid thiolysis. This compound had an EC50 for vasorelaxation of 0.67±0.04 μg/ml when tested for endothelium-dependent relaxing activity in phenylephrine pre-contracted rat aortic rings.
Application Number
10/605,787
Recommended Citation
Fitzpatrick, David F. and O'Malley, Rebecca, "Isolation of a dimer di-gallate, a potent endothelium-dependent vasorelaxing compound" (2006). USF Patents. 672.
https://digitalcommons.usf.edu/usf_patents/672
Assignees
University of South Florida
Filing Date
10/27/2003
Primary/U.S. Class
514/456
