Provided herein is a method for detecting mutations in polynucleotide sequences through the use of a glassy carbon electrode (GCE) coated with a chitosan-graphene nanosheet material (also referred to herein as a “CMG electrode.”) It is a surprising finding of the present invention that the CMG electrodes can be used to detect non-hybridization of capture and target polynucleotides using voltammetry, wherein non-hybridization indicates a mutation or difference in the target polynucleotide as compared to a control.
Mohapatra, Shyam S.; Cissell, Kyle A.; and Alwarappan, Subbiah, "Chitosan-graphene biosensors and methods for their use" (2015). USF Patents. 40.
University of South Florida