Authors

My Lien Dao

Document Type

Patent

Publication Date

December 2013

Patent Number

8609365

Abstract

The present invention provides a method for the staining of fungi and microsporidia for observation with a light microscope based upon the presence of chitin in the composition of these organisms. With the method of the present invention a sample to be analyzed is treated with a solution of Ponceau S and Stains-all dye. The sample is then selectively decolorized and rinsed. The resulting sample is examined with a light microscope, or photographed for a permanent record, to identify the presence of a variety of microorganisms, to include fungi and microsporidia.

Application Number

11/535,564

Assignees

University of South Florida

Filing Date

09/27/2006

Primary/U.S. Class

435/405

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