Document Type
Patent
Publication Date
December 2014
Patent Number
8921312
CPC
A61K 31/375(20130101), A61K 31/665(20130101), A61K 35/28(20130101)
Abstract
Provided is a method of stimulating collagen synthesis and proteoglycan (lumican and keratocan) accumulation. Collagenase isolated keratocytes were cultured with or without insulin with or without ascorbate. Insulin stimulates the synthesis of collagen but does not affect the accumulation of lumican and keratocan. Insulin plus ascorbate, however, stimulates the synthesis of collagen and increased the accumulation of these proteoglycans. The accumulation of PGDS, a KSPG that does not interact with collagen, is not affected by ascorbate. Only the collagen made in the presence of ascorbate was pepsin resistant. EDB overrode the effects of ascorbate on pepsin resistance and proteoglycan accumulation.
Application Number
12/904,489
Recommended Citation
Musselmann, Kurt; Hassell, John R.; Kane, Brad; and Alexandrou, Bridgette, "Combination of insulin and ascorbate to enhance wound healing" (2014). USF Patents. 184.
https://digitalcommons.usf.edu/usf_patents/184
Assignees
University of South Florida
Filing Date
10/14/2010
Primary/U.S. Class
514/59
