Document Type
Patent
Publication Date
December 2014
Patent Number
8916343
CPC
G01N 33/56983(20130101), C12Q 1/6825(20130101), C12Q 1/6837(20130101), G01N 33/5308(20130101)
Abstract
The subject invention concerns materials and methods for detecting nucleic acid sequences. One aspect of the invention concerns a silicon-based “biochip” comprising nucleic acid immobilized thereon. In one embodiment, the silicon comprises microcavities. The nucleic acid to be assayed for the presence of one or more target nucleic acid sequences is immobilized on the silicon. A nucleic acid, such as an oligonucleotide probe, having a sequence substantially complementary to the target nucleic acid sequence can be used to detect the immobilized nucleic acid on the silicon. If the nucleic acid used for detection hybridizes with a target nucleic acid sequence, the hybridized sequences can be detected directly or indirectly. In an exemplified embodiment, the oligonucleotide probe can be labeled with a detectable label, for example, a fluorescent molecule. The subject invention also concerns methods for detecting a target nucleic acid using a silicon-based biochip of the invention.
Application Number
12/931,116
Recommended Citation
Kumar, Arun; Kumar, Ashok; Singh, Shree R.; and Zekri, Souheil, "DNA biochip and methods of use" (2014). USF Patents. 180.
https://digitalcommons.usf.edu/usf_patents/180
Assignees
University of South Florida Alabama State University
Filing Date
01/24/2011
Primary/U.S. Class
435/61
