Notable Sequence Homology of the ORF10 Protein Introspects the Architecture of SARS-CoV-2

Authors

Sk. Sarif Hassan, Pingla Thana Mahavidyalaya
Diksha Attrish, University of Delhi
Shinjini Ghosh, University of Calcutta
Pabitra Pal Choudhury, Indian Statistical Institute, Kolkata
Vladimir N. Uversky, University of South FloridaFollow
Alaa A.A. Aljabali, Yarmouk University-Faculty of Pharmacy
Kenneth Lundstrom, PanTherapeutics, Rte de Lavaux 49, CH1095 Lutry
Bruce D. Uhal, Michigan State University
Nima Rezaei, Tehran University of Medical Sciences
Murat Seyran, University of Vienna
Damiano Pizzol, Italian Agency for Development Cooperation - Khartoum
Parise Adadi, University of Otago, Dunedin
Antonio Soares, University of Texas Health Science Center at San Antonio
Tarek Mohamed Abd El-Aziz, CSIR-Indian Institute of Chemical Technology
Ramesh Kandimalla, Ulster University
Murtaza M. Tambuwala, Patna University
Gajendra Kumar Azad, Tulane University
Samendra P. Sherchan, Federal University of Rio de Janeiro (UFRJ)
Wagner Baetas-da-Cruz, Mayo Clinic, Rochester
Amos Lal, University of Padova
Giorgio Palù, Kyoto University
Kazuo Takayama, Universidad Católica de Valencia
Ángel Serrano-Aroca, Institute of Integrative Omics and Applied Biotechnology (IIOAB)
Debmalya Barh, University of Pittsburgh School of Medicine
Adam M. Brufsky, Universal Scientific Education and Research Network (USERN)

Document Type

Article

Publication Date

6-2021

Keywords

ORF10, SARS-CoV-2, Pangolin-CoV-2020, Mutations, Intrinsic disorder, COVID-19

DOI

https://doi.org/10.1016/j.ijbiomac.2021.03.199

Abstract

The current Coronavirus Disease 19 (COVID-19) pandemic, caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) shows similar pathology to MERS and SARS-CoV, with a current estimated fatality rate of 1.4%. Open reading frame 10 (ORF10) is a unique SARS-CoV-2 accessory protein, which contains eleven cytotoxic T lymphocyte (CTL) epitopes each of nine amino acids in length. Twenty-two unique SARS-CoV-2 ORF10 variants have been identified based on missense mutations found in sequence databases. Some of these mutations are predicted to decrease the stability of ORF10 in silico physicochemical and structural comparative analyses were carried out on SARS-CoV-2 and Pangolin-CoV ORF10 proteins, which share 97.37% amino acid (aa) homology. Though there is a high degree of ORF10 protein similarity of SARS-CoV-2 and Pangolin-CoV, there are differences of these two ORF10 proteins related to their sub-structure (loop/coil region), solubility, antigenicity and shift from strand to coil at aa position 26 (tyrosine). SARS-CoV-2 ORF10, which is apparently expressed in vivo since reactive T cell clones are found in convalescent patients should be monitored for changes which could correlate with the pathogenesis of COVID-19.

Comments

Article available for free at PubMed Central: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051021/

Citation / Publisher Attribution

International Journal of Biological Macromolecules, v. 181, p. 801-809

Scholar Commons Citation

Hassan, Sk. Sarif; Attrish, Diksha; Ghosh, Shinjini; Choudhury, Pabitra Pal; Uversky, Vladimir N.; Aljabali, Alaa A.A.; Lundstrom, Kenneth; Uhal, Bruce D.; Rezaei, Nima; Seyran, Murat; Pizzol, Damiano; Adadi, Parise; Soares, Antonio; Abd El-Aziz, Tarek Mohamed; Kandimalla, Ramesh; Tambuwala, Murtaza M.; Azad, Gajendra Kumar; Sherchan, Samendra P.; Baetas-da-Cruz, Wagner; Lal, Amos; Palù, Giorgio; Takayama, Kazuo; Serrano-Aroca, Ángel; Barh, Debmalya; and Brufsky, Adam M., "Notable Sequence Homology of the ORF10 Protein Introspects the Architecture of SARS-CoV-2" (2021). All publications. 110.
https://digitalcommons.usf.edu/usf_fcrc_all/110