Title

Effects of MDMA (“Ecstasy”) during Adolescence on Place Conditioning and Hippocampal Neurogenesis

Document Type

Article

Publication Date

1-1-2009

Keywords

Adolescent, MDMA, Conditioned place preference, Hippocampus, Proliferation, Neurogenesis, Flow cytometry, Development

Digital Object Identifier (DOI)

https://doi.org/10.1016/j.ejphar.2009.11.017

Abstract

The use of 3,4,methylenedioxymethamphetamine (MDMA), the active agent in ecstasy, during adolescence is widespread yet the effects on adolescent behavior and brain development are unknown. The aim of the present study was 1) to evaluate effects of MDMA in adolescent rats using the conditioned place preference (CPP) paradigm to measure MDMA-induced reward and 2) assess effects of MDMA administration on cellular proliferation, survival and neurogenesis in the dentate gyrus of the hippocampus. During the adolescent period, MDMA CPP was measured in adolescents [postnatal day (PND) 28–39] by training rats to associate 1.25, 2.5, 5.0 mg/kg MDMA or saline administration with environmental cues. After CPP ended, bromodeoxyuridine (BrdU) was injected and rats were euthanized either 24 h (to evaluate cell proliferation) or 2 weeks (to assess neurogenesis) after the last MDMA injection. Adolescents expressed a CPP for 2.5 mg/kg MDMA. Repeated exposure to 5.0 mg/kg MDMA during adolescence increased cell proliferation, yet diminished neurogenesis, an effect that was replicated using flow cytometry. These findings suggest differential dose effects of adolescent MDMA exposure on reward related behaviors and hippocampal neurogenesis.

Was this content written or created while at USF?

Yes

Citation / Publisher Attribution

European Journal of Pharmacology, v. 628, issues 1-3, p. 96-103

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