Title

Tip-enhanced Raman Scattering of DNA Aptamers for Listeria monocytogenes

Document Type

Article

Publication Date

2018

Digital Object Identifier (DOI)

https://doi.org/10.1116/1.5022303

Abstract

Optical detection and conformational mapping of aptamers are important for improving medical and biosensing technologies and for better understanding of biological processes at the molecular level. The authors investigate the vibrational signals of deoxyribonucleic acid aptamers specific to Listeria monocytogenes immobilized on gold substrates using tip-enhanced Raman scattering (TERS) spectroscopy and nanoscale imaging. The authors compare topographic and nano-optical signals and investigate the fluctuations of the position-dependent TERS spectra. They perform spatial TERS mapping with 3 nm step size and discuss the limitation of the resulting spatial resolution under the ambient conditions. TERS mapping provides information about the chemical composition and conformation of aptamers and paves the way to future label-free biosensing.

Was this content written or created while at USF?

Yes

Citation / Publisher Attribution

Biointerphases, v. 13, art. 03C402

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