Title

Cardiac Wound Healing Post-Myocardial Infarction: a Novel Method to Target Extracellular Matrix Remodeling in the Left Ventricle

Document Type

Book Chapter

Publication Date

1-1-2013

Keywords

Cardiac wound healing, Decellularization, Extracellular matrix, Inflammation, Matrix metalloproteinases, Mice, Myocardial infarction

Digital Object Identifier (DOI)

https://doi.org/10.1007/978-1-62703-505-7_18

Abstract

Myocardial infarction (MI) is a leading cause of death worldwide. Permanent ligation of the left anterior descending coronary artery (LAD) is a commonly used surgical model to study post-MI effects in mice. LAD occlusion induces a robust wound healing response that includes extracellular matrix (ECM) remodeling. This chapter provides a detailed guide on the surgical procedure to permanently ligate the LAD. Additionally, we describe a prototype method to enrich cardiac tissue for ECM, which allows one to focus on ECM remodeling in the left ventricle following surgically induced MI in mice.

Was this content written or created while at USF?

No

Citation / Publisher Attribution

Cardiac Wound Healing Post-Myocardial Infarction: a Novel Method to Target Extracellular Matrix Remodeling in the Left Ventricle, in R. Gourdie & T. Myers (Eds.), Wound Regeneration and Repair. Methods in Molecular Biology (Methods and Protocols), Springer, v. 1037, p. 313-324

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