Document Type

Article

Publication Date

6-1-2013

Keywords

Fish oil, inflammation, kidney disease, lifespan, lupus, survival

Digital Object Identifier (DOI)

https://doi.org/10.1177/1535370213489485

Abstract

A growing number of reports indicate that anti-inflammatory actions of fish oil (FO) are beneficial against systemic lupus erythematosus (SLE). However, the majority of pre-clinical studies were performed using 5-20% FO, which is higher than the clinically relevant dose for lupus patients. The present study was performed in order to determine the effective low dose of FDA-approved concentrated FO (Lovaza®) compared to the commonly used FO-18/12 (18-Eicosapentaenoic acid [EPA]/12-Docosahexaenoic acid [DHA]). We examined the dose-dependent response of Lovaza® (1% and 4%) on an SLE mouse strain (NZBxNZW)F1 and compared the same with 1% and 4% placebo, as well as 4% FO-18/12, maintaining standard chow as the control. Results show for the first time that 1% Lovaza® extends maximal lifespan (517 d) and 4% Lovaza® significantly extends both the median (502 d) and maximal (600 d) life span of (NZBxNZW)F1 mice. In contrast, FO-18/12 extends only median lifespan (410 d) compared to standard chow diet (301 d). Additionally, 4% Lovaza® significantly decreased anti-dsDNA antibodies, reduced glomerulonephritis and attenuated lipopolysaccharide-induced pro-inflammatory cytokines (IL-1β, IL-6, TNF-α) in splenocytes compared to placebo. 4% Lovaza® was also shown to reduce the expression of inflammatory cytokines, including IL-1β, IL-6 and TNF-α, while increasing renal anti-oxidant enzymes in comparison to placebo. Notably, NFκB activation and p65 nuclear translocation were lowered by 4% Lovaza® compared to placebo. These data indicate that 1% Lovaza® is beneficial, but 4% Lovaza® is more effective in suppressing glomerulonephritis and extending life span of SLE-prone short-lived mice, possibly via reducing inflammation signaling and modulating oxidative stress.

Was this content written or created while at USF?

No

Citation / Publisher Attribution

Experimental Biology and Medicine, v. 238, issue 6, p. 610-622

Halade, G. V., Williams, P. J., Veigas, J. M., Barnes, J. L., Fernandes, G., Concentrated Fish Oil (Lovaza®) Extends Lifespan and Attenuates Kidney Disease in Lupus-Prone Short-Lived (Nzbxnzw)F1 Mice. Experimental Biology and Medicine, 238(6), pp. 610-622. Copyright © 2013 by SAGE Publications. The final authenticated version is available online at: https://doi.org/10.1177/1535370213489485.

Download

Share

COinS