Volume 49, Issue 3 (2020)



Bubble trail and folia in cenote Zapote, Mexico: petrographic evidence for abiotic precipitation driven by CO2 degassing below the water table
Rafael López-Martínez, Fernando Gázquez, José M. Calaforra, Philippe Audra, Jean Y. Bigot, Teresa Pi Puig, Rocío J. Alcántara-Hernández, Ángel Navarro, Philippe Crochet, Liliana Corona Martínez, and Raquel Daza Brunet

  • The origin of folia is still controversial
  • In the Cenote Zapote occurs the biggest reported folia
  • Folia are the result of subaqueous calcite precipitation around CO2 bubbles


Stratigraphical influence on chalk cave development in Upper Normandy, France: implications for chalk hydrogeology
Daniel Ballesteros, Andrew Farrant, Carole Nehme, Mark Woods, Dominique Todisco, and Damase Mouralis

  • The role of stratigraphic discontinuities on chalk speleogenesis has been analyzed
  • The analyses combine cave geometry, karst geomorphology and stratigraphical sections
  • Chalk caves and karst springs are related to hardgrounds, sheet-flints and marl beds
  • Hardgrounds, sheet-flints and marl beds concentrated groundwater flow forming caves
  • The results reinforce the visualization of the Chalk as a karst aquifer


The impact of sample processing and media chemistry on the culturable diversity of bacteria isolated from a cave
Katey E. Bender, Katelyn Glover, Alexander Archey, and Hazel A. Barton

  • A “culturomics” cultivation study was performed in a cave for the first time
  • Cultivation strategies were assessed for their performance with cave microbes
  • Sample suspension buffer had no effect on culturability
  • Including antibiotics in culture media decreased apparent culturability, diversity
  • Antibiotics enriched rarer slow-growing species that are more difficult to isolate


Distribution of crickets (Subfamily: Phalangopsinae) in caves of Baratang Island, Andaman and Nicobar Islands, India
Amruta Dhamorikar, Dhanusha Kawalkar, and Shirish Manchi

  • Cricket occupancy in the caves is seasonal and zonal with detectability ≤1
  • Relative density and relative abundance does not vary between the caves zones
  • Morphology of the caves influence the abundance and density of crickets
  • Cave with plausible source population with no extinction probability is identified
  • Further studies recommended for the conservation of caves and cave fauna


Urine volume reduction during long-duration cave exploration by a light-weight and portable forward osmosis system
Sebastian Engelhardt, Katey E. Bender, Jörg Vogel, Stephen E. Duirk, Francisco B. Moore, and Hazel A. Barton

  • Forward osmosis (FO) was tested as a strategy for urine mitigation during cave exploration
  • Urea rejection was determined for a novel aquaporin-based membrane (ABM) module
  • A light-weight and portable FO prototype was introduced and tested
  • Urine volume could be reduced by over 80% using the proposed system
  • Draw solution recovery without nitrogen loss could be achieved by evaporation