Influence of Pressure and Dispersant on Oil Biodegradation by a Newly Isolated Rhodococcus Strain from Deep-Sea Sediments of the Gulf of Mexico
Document Type
Article
Publication Date
1-1-2020
Keywords
Biodegradation, Environmental, Geologic Sediments, Gulf of Mexico, Petroleum, Petroleum Pollution, Rhodococcus, Seawater, Water Microbiology
Digital Object Identifier (DOI)
https://doi.org/10.1016/j.marpolbul.2019.110683
Abstract
A new Rhodococcus strain, capable of degrading crude oil, was isolated from the Gulf of Mexico deep-sea sediment and was investigated for its biodegradation characteristics under atmospheric as well as under deep-sea pressure (1500 m = 15 MPa). Additionally, the effect of dispersant (Corexit EC9500A) addition was studied. Rhodococcus sp. PC20 was shown to degrade 60.5 ± 10.7% of the saturated and aromatic fraction of crude oil at atmospheric pressure and 74.2 ± 9.1% at deep-sea level pressure within 96 h. Degradation rates, especially for monoaromatic hydrocarbons, were significantly higher at elevated pressure compared to atmospheric pressure. This study found a growth inhibiting effect at a dispersant to oil ratio of 1:100 and higher. This effect of the dispersant was enhanced when elevated pressure was applied.
Was this content written or created while at USF?
No
Citation / Publisher Attribution
Marine Pollution Bulletin, v. 150, art. 110683
Scholar Commons Citation
Hackbusch, Steffen; Noirungsee, Nuttapol; Viamonte, Juan; Sun, Xiaoxu; Bubenheim, Paul; Kostka, Joel E.; Müller, Rudolf; and Liese, Andreas, "Influence of Pressure and Dispersant on Oil Biodegradation by a Newly Isolated Rhodococcus Strain from Deep-Sea Sediments of the Gulf of Mexico" (2020). C-IMAGE Publications. 36.
https://digitalcommons.usf.edu/cimage_pubs/36
Comments
Data used in this article are available for download.
Crude oil degradation performance of a deep sea Rhodococcus PC20 strain under the influence of elevated hydrostatic pressure and dispersant