A method for facilitating a delivery of a molecule into an interior space of a cell includes the steps of introducing a molecule into a biological structure comprising a cell and applying a substantially continuous low-level electric field, in the form of non-thermal plasma (ionized gas) generated by a direct current voltage applied to an electrode, to the molecule and biological structure. The field is applied for a duration sufficient to effect a change in porosity the cell of the biological structure sufficient to facilitate an entry of a desired molecule into an interior thereof.
Jaroszeski, Mark; Lopez-Diaz, Gabriel A.; Connolly, Richard J.; and Hoff, Andrew M., "Method to facilitate directed delivery and electroporation using a charged stream" (2013). USF Patents. 220.
University of South Florida