Document Type
Patent
Publication Date
June 2014
Patent Number
8748172
Abstract
Provided herein, is a means for development of ferrochelatase variants with improved tolerance towards N-methyl protoporphyrin. Also disclosed are cell assay systems utilizing the variants, as the variants would confer resistance to N-methyl protoporphyrin inhibition and thereby keep heme synthesis uninterrupted. The variants contain loop mutations that affect the NMPP-ferrochelatase interaction, and different degrees of NMPP tolerance are obtained with the introduction of loop mutations in wild-type ferrochelatase. Also disclosed is kinetic mechanism of inhibition of ferrochelatase by NMPP, using the disclosed variants whose mutations in the “porphyrin-interacting loop” motif weakened the potency of NMPP as an inhibitor.
Application Number
14/033,012
Recommended Citation
Ferreira, Gloria C. and Shi, Zhen, "Cell modeling of heme deficiency using ferrochelatase mutations" (2014). USF Patents. 124.
https://digitalcommons.usf.edu/usf_patents/124
Assignees
University of South Florida
Filing Date
09/20/2013
Primary/U.S. Class
435/325
