Presentation Type

Poster

Presenter Information

Nida MahidFollow

Title of Abstract

Epigenetic Regulation in Endophytic Fungi Acquired from Mangrove Trees

Abstract

Gene regulation gives the cell control over structure and function. Epigenetics is an upcoming concept which studies chemical changes DNA without actually changing the genotype. Direct DNA cytosine methylation is associated with strong silencing of gene clusters in fungi. Studies show that a variety of new compounds can be acquired from fungi once those silent bio-synthetic pathways are active. To study the effects of Azacitidine, fungal samples were isolated and cultured from Floridian mangroves collected in Everglades City, Florida. We hypothesized that if we expose our three samples to Azacitidine, it would activate previously silent bio-synthetic pathways thus producing diverse array of secondary metabolites when compared to the unmodified controls. LCMS data confirmed our hypothesis. Results of the modified samples had different peaks when compared to the unmodified controls.

Categories

Natural Sciences

Research Type

Course Related

Mentor Information

Dr. Bill Baker

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Epigenetic Regulation in Endophytic Fungi Acquired from Mangrove Trees

Gene regulation gives the cell control over structure and function. Epigenetics is an upcoming concept which studies chemical changes DNA without actually changing the genotype. Direct DNA cytosine methylation is associated with strong silencing of gene clusters in fungi. Studies show that a variety of new compounds can be acquired from fungi once those silent bio-synthetic pathways are active. To study the effects of Azacitidine, fungal samples were isolated and cultured from Floridian mangroves collected in Everglades City, Florida. We hypothesized that if we expose our three samples to Azacitidine, it would activate previously silent bio-synthetic pathways thus producing diverse array of secondary metabolites when compared to the unmodified controls. LCMS data confirmed our hypothesis. Results of the modified samples had different peaks when compared to the unmodified controls.