Free amino acids of Darlingtonea kentuckensis were investigated by two-dimensional, thin-layer chromatography on Silica Gel G. Thirteen amino acids which could be identifìed (alanine, glutamic acid, glycine, histidine, isoleucine and/or leucine, lysine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine and valine) and seven unidentified ninhydrin-positive spots were found. Beta-Alanine, alpha-amino-butyric acid, arginine, aspartic acid, cystine, hydroxyproline and methionine were not detected. No difference was observed in the free amino acids with respect to sex of beetle, time after feeding and method of sample preparation.
Sperka, R. J..
Free amino acids in a cave beetle Darlingtonea kentuckensis Valentine (Coleoptera: Carabidae).
International Journal of Speleology,
Available at: http://scholarcommons.usf.edu/ijs/vol6/iss3/6