Graduation Year

2005

Document Type

Thesis

Degree

M.S.

Degree Granting Department

Physics

Major Professor

Dr. Chun Min Lo.

Keywords

Invasion, Ecis, Huvecs, Metastasis, Electrode-array

Abstract

The interactions between neighboring cells and between cells and their attached substrate have long been studied in tissue culture. These in vitro studies may provide information regarding cell behavior in vivo including cell movement, cell proliferation, tissue development and wound healing. Transcellular resistance (or impedance) measurements, using various dc or ac techniques have been used to study the barrier function of epithelial and endothelial cell layers. With an appropriate equivalent circuit used for data analysis, junctional resistance between cells and other cellular properties, including cell membrane capacitance, can be determined. However, these techniques have seldom been applied to fibroblastic cell layers because the transcellular resistance is so small that it is difficult to measure it accurately. This research is based on detecting the invasive activities of metastatic cells in vitro using electric cell-impedance sensing (ECIS).

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