Degree Granting Department
Li-June Ming, Ph.D.
Brian T. Livingston, Ph.D.
Kinetics, Reactive oxygen species, Metallopeptide, Amyloid, Enzymology, Sea urchin, Metalloprotease
Although transition metals are essential for life, misregulation of redox-active metal uptake, delivery, storage, and excretion has been linked with a series of neurodegenerative disorders. Alzheimer's disease (AD) is considered an epidemic and is the most widespread of all forms of dementia. Copper ions found in large concentrations localized in amyloid-ß plaques in the brain of AD patients have been linked with the generation of reactive oxygen species which are suspected to be the culprits leading to neuronal cell death. Herein a series of mechanistic and spectroscopic studies elucidate the chemistry about the metal-centered oxidation of biomolecules, including catecholamine neurotransmitters and some analogues by copper-complexes of amyloid-ß peptide. Transition metals can also be useful tools for characterization of metalloproteins due to their unique chemical and spectroscopic features. Herein a series of studies of the native Zn²+ and Cu²+-derivative of recombinant Blastula Protease 10 (BP10) from the sea urchin Paracentrotus lividus are presented in order to elucidate its catalytic mechanism, with the use of enzymology, metal substitution, and electronic absorption spectroscopy.
Scholar Commons Citation
Da Silva, Giordano Faustini Zimmerer, "The bioinorganic chemistry of copper-containing systems: From Type-3 systems pertinent to Alzheimer's disease to mononuclear hydrolysis involved in biological development" (2007). Graduate School Theses and Dissertations.