Graduation Year

2016

Document Type

Thesis

Degree

M.S.M.S.E.

Degree Name

MS in Materials Science and Engineering (M.S.M.S.E)

Degree Granting Department

Chemical Engineering

Major Professor

Venkat Bhethanabotla, Ph.D.

Committee Member

John Kuhn, Ph.D.

Committee Member

Anna Pyayt, Ph.D.

Keywords

Silver Nanocubes, CEA, Immunofluorescence, Biotechnology, Limit of Detection

Abstract

In this paper, metal-enhanced fluorescence (MEF) technique is used to lower the detection limit of carcinoembryonic antigen (CEA) which is able to be utilized in forecasting the risk of having certain kinds of cancers, especially colon and rectal cancer. By incubating silver nanocubes (Ag NCs) on the surface of the chips, the detection limit goes down to below 1ng/mL of CEA. Also, when combining MEF with surface acoustic wave (SAW) devices, the incubation time between antigen and antibody will decrease significantly with the fluorescence signal keeping similar or higher level.

Available for download on Wednesday, November 15, 2017

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