Graduation Year
2011
Document Type
Dissertation
Degree
Ph.D.
Degree Granting Department
Biology (Cell Biology, Microbiology, Molecular Biology)
Major Professor
Jiandong Chen, Ph.D.
Committee Member
Kenneth L. Wright, Ph.D.
Committee Member
Alvaro Monteiro, Ph.D.
Committee Member
Jia Fang, Ph.D.
Keywords
MDMX, ARF, SUV39H1, conformation, Bortezomib
Abstract
MDM2 regulates p53 predominantly by promoting p53 ubiquitination. However, ubiquitination-independent mechanisms of MDM2 have also been implicated. Here we show that MDM2 inhibits p53 DNA binding activity in vitro and in vivo. MDM2 binding promotes p53 to adopt a mutant-like conformation, losing reactivity to antibody Pab1620, while exposing the Pab240 epitope. The acidic domain of MDM2 is required to induce p53 conformational change and inhibit p53 DNA binding. ARF binding to the MDM2 acidic domain restores p53 wild type conformation and rescues DNA binding activity. Furthermore, histone methyl transferase SUV39H1 binding to the MDM2 acidic domain also restores p53 wild type conformation and allows p53-MDM2-SUV39H1 complex to bind DNA. These results provide further evidence for an ubiquitination-independent mechanism of p53 regulation by MDM2, and reveal how MDM2-interacting repressors gain access to p53 target promoters and repress transcription. Furthermore, we show that the MDM2 inhibitor Nutlin cooperates with the proteasome inhibitor Bortezomib by stimulating p53 DNA binding and transcriptional activity, providing a rationale for combination therapy using proteasome and MDM2 inhibitors.
Scholar Commons Citation
Cross, Brittany Lynne, "Inhibition of p53 DNA binding function by the MDM2 acidic domain" (2011). USF Tampa Graduate Theses and Dissertations.
https://digitalcommons.usf.edu/etd/3050
Included in
American Studies Commons, Cell Biology Commons, Molecular Biology Commons, Oncology Commons
