Graduation Year

2004

Document Type

Thesis

Degree

M.S.

Degree Granting Department

Physics

Major Professor

Myung K. Kim

Keywords

microscope, cells, optics, biophysics, hologram

Abstract

We apply the techniques of digital holography to obtain microscopic 3D images of biological cells. Both the amplitude and phase images are obtained from a single hologram, with approximately 1 [mu]m lateral and 10 nm longitudinal resolution. The results are combined with previous experiments resolving the 2 [pi] ambiguity to produce continuous phase images of the samples.

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