σ(N) -Dependent Control of Acid Resistance and the Locus of Enterocyte Effacement in Enterohemorrhagic Escherichia Coli is Activated by Acetyl Phosphate in a Manner Requiring Flagellar Regulator FlhDC and the σ(S) Antagonist FliZ
Carboxylic Acids, Drug Tolerance, Enterohemorrhagic Escherichia coli, Escherichia coli Proteins, Gene Deletion, Gene Expression Regulation, Bacterial, Glutamates, Organophosphates, Phosphoproteins, Repressor Proteins, Sigma Factor, Trans-Activators, Transcriptional Activation
Digital Object Identifier (DOI)
In enterohemorrhagic Escherichia coli (EHEC), sigma factor N (σN) regulates glutamate-dependent acid resistance (GDAR) and the locus of enterocyte effacement (LEE); discrete genetic systems that are required for transmission and virulence of this intestinal pathogen. Regulation of these systems requires nitrogen regulatory protein C, NtrC, and is a consequence of NtrC-σN-dependent reduction in the activity of sigma factor S (σS). This study elucidates pathway components and stimuli for σN-directed regulation of GDAR and the LEE in EHEC. Deletion of fliZ, the product of which reduces σS activity, phenocopied rpoN (σN) and ntrC null strains for GDAR and LEE control, acid resistance, and adherence. Upregulation of fliZ by NtrC-σN was shown to be indirect and required an intact flagellar regulator flhDC. Activation of flhDC by NtrC-σN and FlhDC-dependent regulation of GDAR and the LEE was dependent on σN-promoter flhDP2, and a newly described NtrC upstream activator sequence. Addition of ammonium chloride significantly altered expression of GDAR and LEE, acid resistance, and adherence, independently of rpoN, ntrC, and the NtrC sensor kinase, ntrB. Altering the availability of NtrC phosphodonor acetyl phosphate by growth without glucose, with acetate addition, or by deletion of acetate kinase ackA, abrogated NtrC-σN-dependent control of flhDC, fliZ, GDAR, and the LEE.
This work is licensed under a Creative Commons Attribution 3.0 License.
Was this content written or created while at USF?
Citation / Publisher Attribution
MicrobiologyOpen, v. 3, issue 4, p. 497-512
Scholar Commons Citation
Mitra, Avishek; Fay, Pamela A.; Vendura, Khoury W.; Alla, Zimrisha; Carroll, Ronan K.; Shaw, Lindsey N.; and Riordan, James T., "σ(N) -Dependent Control of Acid Resistance and the Locus of Enterocyte Effacement in Enterohemorrhagic Escherichia Coli is Activated by Acetyl Phosphate in a Manner Requiring Flagellar Regulator FlhDC and the σ(S) Antagonist FliZ" (2014). Cell Biology, Microbiology, and Molecular Biology Faculty Publications. Paper 7.